|Year : 2017 | Volume
| Issue : 3 | Page : 282-286
Susceptibility status of wild population of Phlebotomus sergenti (Diptera: Psychodidae) to different imagicides in a endemic focus of cutaneous leishmaniasis in northeast of Iran
Kourosh Arzamani1, Hassan Vatandoost2, Yavar Rassi3, Mohammad Reza Abai3, Amir Ahmad Akhavan3, Mohammad Alavinia4, Kamran Akbarzadeh3, Mehdi Mohebali5, Sayena Rafizadeh6
1 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran; Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
2 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences; Department of Environmental Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Silences, Tehran, Iran
3 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4 Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran; Toronto Rehabilitation Centre, University Health Network, Toronto, Canada
5 Department of Medical Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
6 Ministry of Health and Medical Education, National Institute for Medical Research Development (NIMAD), Tehran, Iran
|Date of Submission||15-Mar-2017|
|Date of Acceptance||28-Aug-2017|
|Date of Web Publication||7-Nov-2017|
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran
Source of Support: None, Conflict of Interest: None
Background & objectives: Phlebotomine sandflies (Diptera: Psychodidae) transmit several important zoonotic diseases to humans and leishmaniasis is one of them. Two types of leishmaniasis, viz. visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are endemic in Iran. The main vector of anthroponotic cutaneous leishmaniasis (ACL) is Phlebotomus sergenti. The aim of the present study was to determine the susceptibility status of wild strain of P. sergenti to different imagicides of DDT, bendiocarb and permethrin at the median lethal time, LT50 level.
Methods: Sandflies were collected from selected village in North Khorasan Province, northeast of Iran from indoors using CDC light-traps. Susceptibility test was carried out against DDT (4%), bendiocarb (0.1%) and permethrin (0.75%) for all the females according to WHO method, and mortality was calculated. Species identification was carried out using the morphological keys. Data were analysed using probit regression analysis to determine the LT50 and LT90 values.
Results: In total, 851 female P. sergenti sandflies were tested. LT50 values to DDT (4%), Bendiocarb (0.1%) and permethrin (0.75%) were 15.4, 19.2 and 6.3 min respectively. The values for LT90 were 51.1, 47.4 and 18.6 min respectively. The mortality rates for 1 h exposure time to DDT, bendiocarb and permethrin were 89.8 ± 1.4; 93.6 ± 1.4; and 95.6 ± 1.7%, respectively.
Interpretation & conclusion: The suscesptibility studies revealed development of resistance against DDT (4%) in the wild strain of P. sergenti population. Monitoring and mapping of insecticide resistance in the region is recommended for vector control.
Keywords: Insecticide resistance; Iran; leishmaniasis; Phlebotomus sergenti
|How to cite this article:|
Arzamani K, Vatandoost H, Rassi Y, Abai MR, Akhavan AA, Alavinia M, Akbarzadeh K, Mohebali M, Rafizadeh S. Susceptibility status of wild population of Phlebotomus sergenti (Diptera: Psychodidae) to different imagicides in a endemic focus of cutaneous leishmaniasis in northeast of Iran. J Vector Borne Dis 2017;54:282-6
|How to cite this URL:|
Arzamani K, Vatandoost H, Rassi Y, Abai MR, Akhavan AA, Alavinia M, Akbarzadeh K, Mohebali M, Rafizadeh S. Susceptibility status of wild population of Phlebotomus sergenti (Diptera: Psychodidae) to different imagicides in a endemic focus of cutaneous leishmaniasis in northeast of Iran. J Vector Borne Dis [serial online] 2017 [cited 2019 Nov 11];54:282-6. Available from: http://www.jvbd.org/text.asp?2017/54/3/282/217621
| Introduction|| |
Phlebotomine sandflies (Diptera: Psychodidae) are important vectors of human leishmaniasis, affecting almost 98 countries of the world. Iran is one of the 10 countries with the highest estimated cases of cutaneous leishmaniasis (CL) in the world. In Iran, two common types of leishmaniases, viz. visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are endemic. Visceral leishmaniasis is endemic in few foci of the country, including North Khorasan Province, and Leishmania infantum has been detected in vectors and reservoirs of the disease in this focus,,. Cutaneous leishmaniasis is endemic in two forms, zoonotic cutaneous leishmaniasis (ZCL) and anthroponotic cutaneous leishmaniasis (ACL). ZCL is distributed in many rural areas of 17 out of 31 provinces (including North Khorasan) in Iran,,. The main vector of ZCL is P. papatasi and L. major is the parasitic disease agent. Anthroponotic cutaneous leishmaniasis is endemic in at least eight provinces of Iran. The main vector of ACL is P. sergenti, and the disease agent is L. tropica, mainly distributed in large and medium-size cities of Iran. More than 2831 cases of CL have been reported from several counties of this province in duration of eight years, from 2006 to 2013.
There are numerous reports on susceptibility status of phlebotomine sandflies to different insecticides in the world, that have used the discriminative dose of pesticides againt malaria vectors, as described by WHO to determine the resistance of sandflies to insecticides,,,,.
Some of these studies indicated that P. sergenti was susceptible to deltamethrin as well as DDT (4%) in the ACL foci in Mashad and Isfahan cities, in northeast and central Iran respectively,. In the ZCL foci, P. papatasi was susceptible to DDT (4%) in Badrood county (central Iran), to DDT (4%), permethrin (0.25%) and propoxur (0.1%) in the Sabzevar (northeast of Iran), and to DDT (4%) in the Orzouiye county (south of Iran),,.
In North Khorasan, DDT (75%) has been used as residual spraying since 1954 for malaria control. In recent years, several pyrethroids, carbamates and organophosphorus compounds are used for veterinary and agricultural pest control in many parts of the province (Ministry of Health, Iran).
Insecticide resistance threatens the effectiveness of control measures, and routine monitoring of insecticide resistance among natural populations of vectors further helps the authorities in early detection of resistance of vectors to insecticides, and improving effectiveness of operational control strategies. The present study was the first attempt to investigate the susceptibility status of phlebotomine sandflies to DDT, bendiocarb and permethrin on the wild population of P. sergenti in North Khorasan Province, Iran based on different exposure times. The LT50 and LT90 values were caculated using probit regression line. The comparison of different insecticides was carried out based on LT50 and LT90 values.
| Material & Methods|| |
This survey was conducted during early-July to late August of 2015–16. Sandflies were collected from the Villages of Kohne Jolgheh, Maneh and Samalqan county, in North Khorasan Province, northeast of Iran where human VL and CL had been reported in the last 5 yr. The province is bordered by Turkmenistan in the north, Khorasan Razavi Province in the east and southeast, Semnan Province in the southwest and Golestan Province in the west. The study area is laid between 36°37'–38°17' N latitude and 55°53'–58°20' E longitude with an area of 28,434 km2. The province comprises of eight counties with a population of 8,63,092 (2016 census). It has desert and mountainous areas and receives about 250 mm of rainfall annually. The City of Bojnurd is the center of the province. Kohne Jolgeh village is located near the Turkmenistan border, 130 km far from Bojnurd City and its population was 2900 in the year 2016.
Sandflies were collected from indoors including human dwelling and livestock shelters, using CDC lighttraps from 2000 till 0200 hrs. The specimen collection was performed in accordance with the procedures approved by the Ethical Committee of North Khorasan University of Medical Sciences. The caught sandflies were transported immediately to the Research Center of Vector-Borne Diseases, North Khorasan University of Medical Sciences, Iran for carrying out the susceptibility tests. Collected sandflies were transferred in a wooden cage with a hanging piece of wet cloth for providing the suitable humidity. They were fed by a small amount of sucrose solution soaked cotton. In total, 851 field sandflies were collected and identified using appropriate keys,,.
Procurement of insecticide impregnated papers and their concentration
Impregnated papers with DDT (4%) (Batch No. DD186, Exp. date: July 2016); permethrin (0.75%) (Batch No. PE289, Exp. date: Sep 2015); and bendiocarb (0.1%) (Batch No. BE106, Exp. date: June 2016), as well as papers for control were supplied by the collaborating center of the World Health Organization in Penag, Malaysia.
Collected sandflies were maintained under laboratory and appropriate climatical condition. Female sandflies were subjected to susceptibility tests according to the guidelines of WHO. Sandflies were exposed to impregnated papers (DDT, bendiocarb, permethrin) at different logarithmic times. They were provided 10% sucrose solution during the recovery period. The mortality was recorded after 24 h of recovery period. After each test, all the dead and alive sand flies were preserved in 96% ethanol for mounting in Puri’s media in order to identify the species using morphological keys,. The exposure time was set between 3.75 and 60 min, and at least 5 log times were selected for estimation of lethal times. Out of the 851 field collected sandflies, 692 were used as treatement and 159 as control.
The exposure time vs probit mortality analysis was carried out according to Finney. The LT50 and LT90 values were estimated and the slope values of the regression lines were calculated.
| Results|| |
In total, 692 female specimens were used for insecticide susceptibility tests. A total of 159 sandflies were used as control. The mortality rate for controls was < 5%, eliminating the requirement of Abbot’s correction.
The results of the tests revealed the LT50 values to DDT (4%), bendiocarb (0.1%) and permethrin (0.75%) were 15.4, 19.2 and 6.3 min and the corresponding LT90 values were 51.1, 47.4 and 18.6 min [Table 1]. The regression lines for mortality of P. sergenti exposed to the three insecticides used are plotted against exposure times [Figure 1]. Mortality rates calculated for P. sergenti after 1 h exposure to DDT (4 %) , bendiocarb (0.1%) and permethrin (0.75% ) were 89.8 ± 1.4% , 93.6 ± 1.4% and 95.6 ± 1.7% respectively [Table 2].
|Table 1: LT50 and LT90 values for P. sergenti to DDT (4%), bendiocarb (0.1%) and permethrin (0.75%), in North Khorasan Province, Iran (2016)|
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|Figure 1: Comparison of regression lines, equations and LT50 of P. sergenti when exposed to DDT (4%), bendiocarb (0.1%) and permethrin (0.75%).|
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|Table 2: Mortality parameters for P. sergenti when exposed to different insecticides using WHO's method in a endemic focus of cutaneous leishmaniasis in North Khorasan Province, northeast of Iran, 2016|
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| Discussion|| |
The susceptibility status of phlebotomine sandflies to DDT and other group of insecticides (bendiocarb, permethrin etc.) has been reported in several studies across the world. In Iran, some investigations have been carried out on P. papatasi but prior to this study, no reports are available on the susceptibility levels of phlebotomine sand flies (P sergenti) from North Khorasan Province, one of the important CL foci in northeast of Iran. The present study is first attempt to reveal the susceptibility status of wild strain of P. sergenti to three conventional insecticides, i.e. DDT, bendiocarb and permethrin in this region.
According to the results of this study, permethrin 0.75% showed highest efficiency against P. sergenti at discriminative dose with mortality rate of 95.6 ± 1.7%, followed by bendiocarb (0.1%) and DDT (4%), with mortality rates of 93.6 ± 1.4% and 89.8 ± 1.4%, respectively.
Resistance in P. papatasi has been reported for the first time in 1979 from Muzaffarpur district of Bihar, India which was later supported in subsequent investigations. In Iran, there are limited studies on insecticide susceptibility of phlebotominae sandflies. The result of these investigations have indicated the susceptibility of P. papatasi to different kinds of insecticide except tolerance to DDT in Isfahan Province, central Iran.
The reports on insecticide susceptibility status of P sergenti are limited and there are no records of insecticide resistance till now. Similar studies in Egypt confirmed the susceptibility of P. langeroni, P. papatasi and P. sergenti to DDT, resmethrin, cyfluthrin, permethrin, bendiocarb and malathion. A study in Morocco, showed that wild populations of P. sergenti and P. papatasi were susceptible to lambdacyhalothrin, DDT and malathion. An earlier study on P. sergenti revealed that this species was susceptible to the deltamethrin and DDT among ACL foci in northeast, central and south Iran,,. All these studies have been conducted, according to the WHO guidelines for malaria vectors at the diagnostic dose. There are specific guidelines approved by WHO for diagnostic dose of mosquitoes, however, there are no such guidelines for phlebotominae sandflies; hence, trials were conducted, according to the results of LT50 and then compared with each other. However, the results of earlier studies were contradictory to this study.
| Conclusion|| |
The permethrin is efficient against P. sergenti at discriminative dose, followed by bendiocarb. For the first time, resistance against DDT was observed in wild strain of P. sergenti population in North Khorasan Province, Iran. These findings can be used as evidence for demonstrating the development of insecticide resistance in sandflies, indicating the necessity of systematic monitoring of resistance in sandfly populations.
| Acknowledgements|| |
This article has been extracted from the Ph.D. thesis submitted by corresponding authors in the Department of Medical Entomology, School of Public Health at Tehran University of Medical Sciences, Tehran, Iran. This study received financial support from the Tehran University of Medical Sciences (Project No. 27811) and Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran (Project No.: 95/60/729).
Conflict of interest
The authors declare that they have no conflict of interest in this study.
| References|| |
Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, et al
. Leishmaniasis worldwide and global estimates of its incidence. PloS One
2012; 7(5): e35671.
Arzamani K. Visceral leishmaniasis in North Khorasan Province, northeast of Iran. Int J Infect Dis
Mohebali M, Arzamani K, Zarei Z, Akhoundi B, Hajjaran H, Raeghi S, et al
. Canine visceral leishmaniasis in wild canines (Fox, Jackal, and Wolf) in northeastern Iran using parasitological, serological, and molecular methods. J Arthropod Borne Dis
2015; 10(4): 538–45.
Rassi Y, Abai M, Oshaghi M, Javadian E, Sanei A, Rafidzadeh S, et al
. First detection of Leishmania infantum
in Phlebotomus kandelakii
using molecular methods in northeastern Islamic Republic of Iran. East Mediterr Health J
Yaghoobi-Ershadi M. Phlebotomine sandflies (Diptera: Psychodidae) in Iran and their role on Leishmania
transmission. J Arthropod Borne Dis
2012; 6(1): 1.
Alavinia S, Arzamani K, Reihani M, Jafari J. Some epidemiological aspects of cutaneous leishmaniasis in Northern Khorasan Province, Iran. J Arthropod Borne Dis
2009; 3(2): 50.
Rajabzadeh RAK, Shoraka HR, Riyhani H, Seyed Hamid Hosseini. Epidemiological survey and geographical distribution of cutaneous Leishmaniasis in North Khorasan Province, 2006–2013. Int J Epidemiol Res
2015; 2(4): 197–203.
Amalraj DD, Sivagnaname N, Srinivasan R. Susceptibility of Phlebotomus argentipes
and P. papatasi
(Diptera: Psychodidae) to insecticides. J Commun Dis
1999; 31(3): 177–80.
Artemiev MM, Gadzhibekova EA, Bondareva NI. Susceptibility of sandflies (Diptera: Psychodidae: Phlebotominae) to DDT in the focus of visceral leishmaniasis in the Azerbaijan SSR. Med Parazitol
(Mosk) 1984; 6:
Bansal S, Singh K. Susceptibility status of Phlebotomus papatasi
and Sergentomyia punjabaensis
(Diptera: Psychodidae) to some insecticides in District Bikaner (Rajasthan). J Commun Dis
1996; 28(1): 28–32.
Das Gupta RK, Saxena NB, Joshi RD, Rao JS. DDT resistance in P. papatasi
in Bihar. J Commun Dis
1995; 27(2): 124.
Denlinger DS, Lozano-Fuentes S, Lawyer PG, Black WC, Bernhardt SA. Assessing Insecticide susceptibility of laboratory Lutzomyia longipalpis
and Phlebotomus papatasi
Sand flies (Diptera: Psychodidae: Phlebotominae). J Med Entomol
Dhiman RC, Yadav RS. Insecticide resistance in phlebotomine sandflies in Southeast Asia with emphasis on the Indian subcontinent. Infect Dis Poverty
2016; 5(1): 106.
Dinesh DS, Das ML, Picado A, Roy L, Rijal S, Singh SP, et al
. Insecticide susceptibility of Phlebotomus argentipes
in visceral leishmaniasis endemic districts in India and Nepal. PLoS Negl Trop Dis
2010; 4(10): e859.
Faraj C, Ouahabi S, Adlaoui EB, El Elkohli M, Lakraa L, El Rhazi M, et al
. Insecticide susceptibility status of Phlebotomus (Paraphlebotomus) sergenti
and Phlebotomus (Phlebotomus) papatasi
in endemic foci of cutaneous leishmaniasis in Morocco. Parasit Vectors
2012; 5(1): 1.
Hassan MM, Widaa SO, Osman OM, Numiary MSM, Ibrahim MA, Abushama HM. Insecticide resistance in the sandfly, Phle- botomus papatasi
from Khartoum State, Sudan. Parasit Vectors
2012; 5(1): 46.
Karakus M, Gocmen B, Özbel Y. Insecticide susceptibility status of wild-caught sandfly populations collected from two leishmaniasis endemic areas in western Turkey. J Arthropod Borne Dis
Lavagino A, Ansaldi G. Susceptibility tests on Phlebotomus perniciosus
and Phlebotomus perfiliewi
wild populations in Sicily. Parassitologia
1991; 33(Suppl): 349–51.
Li AY, Pérz de León AA, Linthicum KJ, Britch SC, Bast JD, Debboun M. Baseline susceptibility to pyrethroid and or- ganophosphate insecticides in two Old World sandfly species (Diptera: Psychodidae). US Army Med Dep J
2015; 2015; Jul- Sep:
Maroli M, Cianchi T, Bianchi R, Khoury C. Testing insecticide susceptibility of Phlebotomus perniciosus
and P. papatasi
(Diptera: Psychodidae) in Italy. Ann Ist Super Sanità
Singh R, Mittal P, Dhiman R. Insecticide susceptibility status of Phlebotomus argentipes
, a vector of visceral leishmaniasis in different foci in three states of India. J Vector Borne Dis
Moosa-Kazemi S, Yaghoobi-Ershadi M, Akhavan A, Abdoli H, Zahraei-Ramazani A, Jafari R, et al
. Deltamethrin-impregnated bednets and curtains in an anthroponotic cutaneous leishmaniasis control program in northeastern Iran. Ann Saudi Med
2007; 27(1): 6.
Zahraei-Ramazani AR Y-EM, Akhavan AA, Abdoli H, Jafari R, Jalali-Zand AR, et al
. Some ecological aspects of phleboto- minae sandflies (Diptera: Psychodidae) in an endemic focus of anthroponotic cutaneous leishmaniasis of Iran. J Entomol
2008; 5(1): 17–23.
Saeidi Z, Vatandoost H, Akhavan AA, Yaghoobi-Ershadi MR, Rassi Y, Sheikh Z, et al
. Baseline susceptibility of a wild strain of Phlebotomus papatasi
(Diptera: Psychodidae) to DDT and pyrethroids in an endemic focus of zoonotic cutaneous leish- maniasis in Iran. Pest Mang Sci
2012; 68(5): 669–75.
Yaghoobi-Ershadi MR, Akhavan AA, Abai MR, Ebrahimi B, Zahraei-Ramazani AR, Vafaei-Nezhad R, Hanafi-Bojd AA, Jafari R. Epidemiological study in a new focus of cutaneous leishmaniasis in the Islamic Republic of Iran. East Mediterr Health J
2004; 70(4–5): 688.
Yaghoobi-Ershadi M, Akhavan A. Entomological survey of sandflies (Diptera: Psychodidae) in a new focus of zoonotic cutaneous leishmaniosis in Iran. Acta Trop
1999; 73(3): 321–6.
|27.|Test procedures for insecticide resistance monitoring in malaria vector mosquitoes
, II edn. Geneva: World Health Organization 2016. Available from: http://apps.who.int/iris/bitstre am/10665/250677/1/9789241511575-eng.pdf
(Accessed on August 15, 2017).
Seyedi-Rashti MA, Nadim A. The genus Phlebotomus (Diptera: Psychodidae: Phlebotominae) of the countries of the eastern Mediterranean region. Iranian J Pub Health
1992; 21(1–4): 11–50.
Theodor O, Mesghali A. On the phlebotominae of Iran. J Med Entomol
1964; 1(3): 285–300.
Finney DJ. Probit Analysis
, III edn. England: Cambridge University Press 1971; p. 333.
Joshi G, Kaul S, Wattal B. Susceptibility of sandflies to organochlorine insecticides in Bihar (India)—Further reports. J Commun Dis
1979; 11(4): 209–13.
Tetreault GE, Zayed AEBB, Hanafi HA, Beavers GM, Zeichner BC. Susceptibility of sandflies to selected insecticides in North Africa and the Middle East. J Am Mosq Control Assoc
2001; 17(1): 23–7.
Afshar AA, Rassi Y, Sharifi I, Abai M, Oshaghi M, Yaghoobi- Ershadi M, et al
. Susceptibility status of Phlebotomus papatasi
and P. sergenti
(Diptera: Psychodidae) to DDT and deltamethrin in a focus of cutaneous leishmaniasis after earthquake strike in Bam, Iran. J Arthropod Borne Dis
2011; 5(2): 32.
[Table 1], [Table 2]