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  Indian J Med Microbiol
 

Figure 3: Western blot analysis of NT4 gene expression in transgenic Leishmania on Days 3, 7, 10, 15 and 20 after transfection. Lane 1: Protein marker; Lane 2: Mutant strain on Day 20; Lanes 3-6: Mutant strains on Days 15, 10, 7 and 3, respectively post-transfection; Lane 7: Negative control; and Lane 8: Wild strain used as positive control. The result of this experiment shows that protein was produced in transgenic pro- mastigotes just on Day 20 after electroporation; and in other cases, probably there was no gene expression or the expression was too low, as no bands were observed.

Figure 3: Western blot analysis of <i>NT4</i> gene expression in transgenic <i>Leishmania</i> on Days 3, 7, 10, 15 and 20 after transfection. Lane 1: Protein marker; Lane 2: Mutant strain on Day 20; Lanes 3-6: Mutant strains on Days 15, 10, 7 and 3, respectively post-transfection; Lane 7: Negative control; and Lane 8: Wild strain used as positive control. The result of this experiment shows that protein was produced in transgenic pro- mastigotes just on Day 20 after electroporation; and in other cases, probably there was no gene expression or the expression was too low, as no bands were observed.