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RESEARCH ARTICLE
Year : 2019  |  Volume : 56  |  Issue : 3  |  Page : 252-262

Molecular characterization of Trypanosoma cruzi and Leishmania spp. coinfection in mammals of Venezuelan coendemic areas


1 Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana Alonso” (BIOMED); Departamento de Clinico Integral, Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, Venezuela
2 Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela
3 Centro de Investigaciones en Enfermedades Tropicales (CIET-UC), Facultad de Ciencias de la Salud, Universidad de Carabobo, San Carlos, Cojedes, Venezuela
4 Centro de Medicina Tropical de Oriente, Universidad de Oriente (UDO) Núcleo Anzoátegui, Barcelona, estado Anzoátegui, Venezuela
5 Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana Alonso” (BIOMED), Maracay, Venezuela
6 Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana Alonso” (BIOMED); Centro Nacional de Referencia de Flebótomos, BIOMED, Facultad de Ciencias de la Salud, Universidad de Carabobo, Maracay, Venezuela
7 Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana Alonso” (BIOMED); Departamento de Parasitología, Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, estado Aragua, Venezuela

Correspondence Address:
Dr E Ferrer
Instituto de Investigaciones Biomédicas “Dr Francisco J. Triana Alonso” (BIOMED), Universidad de Carabobo Sede Aragua, Maracay
Venezuela
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-9062.289394

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Background & objectives: Trypanosoma cruzi and Leishmania spp. are protozoans that cause American trypanosomiasis and leishmaniasis, respectively. In endemic foci where both diseases coincide, coinfection can occur. The objective of this work was the characterization of the parasites involved in coinfection in several endemic areas of Venezuela. Methods: Molecular characterization was done in 30 samples of several species of mammals (Didelphis marsupialis, Equus mulus, Rattus rattus, Canis familiaris, Felis catus, and Sciurus granatensis) from the states of Anzoategui, Cojedes and Capital District diagnosed with T. cruzi and Leishmania spp. coinfections. For the typing of T. cruzi DTUs, the markers of miniexon, 24Sa rDNA, 18Sa rDNA, and hsp60-PCR-RFLP (EcoRV) were used. Infection by Leishmania spp. was characterized by miniexon multiplex PCR for complexes of Leishmania and ITS1-PCR-RFLP (HaeIII, HhaI, and RsaI) for the identification of the species. Results: The T. cruzi TcI was present in 100% of the coinfected mammals, which included 76.7% of triple infection by T. cruzi TcI-complex–L. (L) mexicana–L. infantum/chagasi, 13.3% of double infection by T. cruzi TcI-L. mexicana and 10% of double infection by T. cruzi Tcl—L. infantum/chagasi. Interpretation & conclusion: These results suggest that the double or triple infection is a phenomenon existing in almost all the coendemics areas and mammals studied, which might influence the mechanisms of adaptation and pathogenicity of these parasites.


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