• Users Online: 425
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
 
RESEARCH ARTICLE
Ahead of Print

Functional studies of Plasmodium falciparum putative SURF1 in Saccharomyces cerevisiae


1 Indian Council of Medical Research - National Institute of Traditional Medicine, Belagavi, Karnataka, India
2 Department of Microbiology, KLE Academy for higher education, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India
3 Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, India
4 Department of Microbiology, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, India
5 Indian Council of Medical Research - National Institute of Traditional Medicine, Belagavi, Karnataka; Department of Epidemiology and Public Health, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, India

Correspondence Address:
Praveen Balabaskaran Nina,
Assistant Professor, Department of Epidemiology and Public Health, Central University of Tamil Nadu, Thiruvarur
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-9062.311773

Background and objectives: The mitochondrial electron transport chain (mtETC) of Plasmodium falciparum is an important drug target. Identification and functional validation of putative mitochondrial proteins of the mtETC is critical for drug development. Many of the regulatory subunits and assembly factors of cytochrome c oxidase that are readily identifiable in humans and yeast are missing in P. falciparum. Here, we describe our efforts to identify and validate the function of putative PfSURF1, a key assembly factor of complex IV of the mtETC. Methods: Multiple sequence alignment was carried out in Clustal X 2.1. Phylogenetic tree was constructed using “Draw tree” option in Clustal X, and was analyzed using interactive Tree of Life software. To identify the conserved sequences, domain search was done using Jalview version 2.8.2 (BLOSUM 62 scoring). The haploid Saccharomyces cerevisiae strain (BY4741) containing the null allele shy1 (Orf: YGR112w) (shy1::Kan) was complemented with putative PfSURF1 to study its ability to rescue the growth defect. Results: Similarity searches of PfSURF1-like proteins in the Pfam shows statistically significant E = 4.7e-10 match to SURF1 family. Sequence alignment of PfSURF1 with other SURF1-like proteins reveals the conservation of transmembrane domains, alpha-helix and beta-pleated sheets. Phylogenetic analysis clusters putative PfSURF1 with apicomplexan SURF1-like proteins. Yeast complementation studies show that PfSURF1 can partially rescue the yeast shy1 mutant, YGR112w. Interpretation & conclusion: Bioinformatics and complementation studies in yeast show that P. falciparum's SURF1 is the functional ortholog of human SURF1 and yeast Shy1.


Print this article
Search
 Back
 
  Search Pubmed for
 
    -  Chellappan S
    -  Roy S
    -  Nagmoti JM
    -  Tabassum W
    -  Vukanti R
    -  Hoti S L
    -  Bhattacharyya MK
    -  Nina PB
 Citation Manager
 Article Access Statistics
 Reader Comments
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed81    
    PDF Downloaded0    

Recommend this journal