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  Indian J Med Microbiol
 

Figure 2: Nested PCR amplification of DNA extracted from sandflies, rodents and human 1: Leishmania major from Sergentomyia dentata. 2, 3 and 6: L. major from Phlebotomus papatasi. 4 and 10: 50 bp DNA ladder (Fermentas). 5: L. major from Sergentomyia clydei. 7: Leishmania major from Nesokia indica. 8 and 9: L. major from Tatera indica. 11: Negative control. 12: L. major positive control. 13 and 15: L. major from human.

Figure 2: Nested PCR amplification of DNA extracted from sandflies, rodents and human 1: <i>Leishmania major</i> from <i>Sergentomyia dentata</i>. 2, 3 and 6: <i>L. major</i> from <i>Phlebotomus papatasi</i>. 4 and 10: 50 bp DNA ladder (Fermentas). 5: <i>L. major</i> from <i>Sergentomyia clydei</i>. 7: <i>Leishmania major</i> from <i>Nesokia indica</i>. 8 and 9: <i>L. major</i> from <i>Tatera indica</i>. 11: Negative control. 12: <i>L. major</i> positive control. 13 and 15: <i>L. major</i> from human.